Simultaneous imaging of multiple focal planes for three-dimensional microscopy using ultra-high-speed adaptive optics.
J Biomed Opt
; 17(5): 050505, 2012 May.
Article
em En
| MEDLINE
| ID: mdl-22612120
ABSTRACT
Traditional white-light and fluorescent imaging techniques provide powerful methods to extract high-resolution information from two-dimensional (2-D) sections, but to retrieve information from a three-dimensional (3-D) volume they require relatively slow scanning methods that result in increased acquisition time. Using an ultra-high speed liquid lens, we circumvent this problem by simultaneously acquiring images from multiple focal planes. We demonstrate this method by imaging microparticles and cells flowing in 3-D microfluidic channels.
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Base de dados:
MEDLINE
Assunto principal:
Acústica
/
Iluminação
/
Aumento da Imagem
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Imageamento Tridimensional
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Lentes
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Microscopia
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article