Phorbol esters stimulate the transport of anionic amino acids in cultured human fibroblasts.
Biochem Biophys Res Commun
; 173(3): 1304-10, 1990 Dec 31.
Article
em En
| MEDLINE
| ID: mdl-2268332
ABSTRACT
The effect of phorbol esters on the transport of amino acids has been evaluated in cultured human fibroblasts. The activity of the Na(+)-dependent system XAG- for anionic amino acids is selectively and markedly stimulated by phorbol esters. The effect is maximal within 15 min; it is attributable to an increase in transport maximum (Vmax) and not prevented by inhibitors of protein synthesis. The half-maximal stimulation is observed at concentrations of phorbol 12,13-dibutyrate lower than 100 nM. Prolonged incubations in the presence of 1 microM phorbol 12,13-dibutyrate lower the binding of the ligand to its receptor with a loss of the stimulatory effect on transport. The results presented indicate that the stimulation of amino acid transport through system XAG- by phorbol esters requires the activation of protein kinase C.
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Base de dados:
MEDLINE
Assunto principal:
Proteína Quinase C
/
Dibutirato de 12,13-Forbol
/
Fibroblastos
/
Aminoácidos
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article