In vivo functional analysis of transcription factor: response element interaction using transgenic Xenopus laevis.

Analysis of transcription factor-target interactions in vivo is important to the study of transcriptional regulation of gene expression. A key experiment involves analysis of the functional interaction between a trans-acting factor and its corresponding cis-acting element in the context of a target promoter in vivo. We describe a method for this analysis in transgenic Xenopus tadpoles in which expression of the trans-acting factor is knocked down using an shRNA-mediated approach.