Retinal transcriptome profiling by directional next-generation sequencing using 100 ng of total RNA.
Methods Mol Biol
; 884: 319-34, 2012.
Article
em En
| MEDLINE
| ID: mdl-22688717
ABSTRACT
RNA expression profiles produced by next-generation sequencing (NGS) technology (RNA-seq) allow comprehensive investigation of transcribed sequences within a cell or tissue. RNA-seq is rapidly becoming more cost-effective for transcriptome profiling. However, its usage will expand dramatically if one starts with low amount of RNA and obtains transcript directionality during the analysis. Here, we describe a detailed protocol for the creation of a directional RNA-seq library from 100 ng of starting total RNA.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Retina
/
Análise de Sequência de RNA
/
Perfilação da Expressão Gênica
/
Sequenciamento de Nucleotídeos em Larga Escala
/
Transcriptoma
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article