Altered endothelin expression in granulosa-lutein cells of women with polycystic ovary syndrome.
Life Sci
; 91(13-14): 703-9, 2012 Oct 15.
Article
em En
| MEDLINE
| ID: mdl-22727793
ABSTRACT
AIMS:
To examine the levels of endothelin system components in granulosa lutein cells (GLCs) of women with PCOS and compare them to normally ovulating women undergoing In Vitro Fertilization (IVF). Polycystic ovary syndrome (PCOS) is one of the most common endocrine-metabolic disorders in women of reproductive age. Endothelins are locally produced by endothelial and granulosa cells of the preovulatory follicle. Abnormal expression or production of endothelins may be a contributing factor in PCOS pathogenesis. MAINMETHODS:
Follicular aspirates containing GLCs were obtained from PCOS and normally ovulating patients undergoing oocyte retrieval during the IVF cycle. RNA was extracted and endothelin system components were quantified using real-time PCR. GLCs were cultured in basal media for 7 days, and then challenged with various luteinizing agents (luteinizing hormone, hCG, or forskolin) for 24 h. KEYFINDINGS:
In GLCs from women with PCOS, Endothelin-1 mRNA expression was elevated (2.2-fold) as compared with normally ovulating women, whereas endothelin-2 mRNA was reduced (1.8-fold). ET receptors and endothelin-converting enzyme showed the same expression levels in the two groups. In vitro modeling showed that although the steroidogenic response was preserved in GLC, endothelin expression levels were not exhibited in vitro in their original pattern.SIGNIFICANCE:
Dysregulation of ovarian endothelin expression may induce a pathologic ovulation pattern characteristic of PCOS.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Síndrome do Ovário Policístico
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Regulação da Expressão Gênica
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Endotelina-1
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Endotelina-2
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Células Lúteas
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article