Biochemical, genetic, and serological characterization of two capsule subtypes among Streptococcus pneumoniae Serotype 20 strains: discovery of a new pneumococcal serotype.
J Biol Chem
; 287(33): 27885-94, 2012 Aug 10.
Article
em En
| MEDLINE
| ID: mdl-22736767
ABSTRACT
The bacterial pathogen Streptococcus pneumoniae expresses one of over 90 structurally distinct polysaccharide (PS) capsule serotypes. Prior PS structural analyses of the vaccine-associated serotype 20 do not agree with reports describing the genes that mediate capsule synthesis. Furthermore, using immunized human sera-based assays, serological differences were recently noted among strains typed as serotype 20. We examined the capsule structures of two serologically dissimilar serotype 20 strains, 20α and 20ß, by extensive biochemical analysis. 20α PS was composed of the previously described serotype 20 hexasaccharide repeat unit, whereas the 20ß PS was composed of a novel heptasaccharide repeat unit containing an extra branching α-glucose residue. Genetic analysis of the subtypes revealed that 20α may have arisen from a 20ß progenitor following loss of function mutation to the glycosyltransferase gene whaF. Conventional serotyping methods using rabbit polyclonal or mouse monoclonal antibodies were unable to distinguish the subtypes. However, genetic analysis of multiple "serotype 20" clinical isolates revealed that all strains contain the 20ß genotype. We propose naming bacteria that express the previously described 20α capsule structure 20A and bacteria that express the novel 20ß capsule structure 20B, a new pneumococcal serotype.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Oligossacarídeos
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Polissacarídeos Bacterianos
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Streptococcus pneumoniae
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Cápsulas Bacterianas
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article