Cell-free serum DNA in patients with bladder cancer: results of a prospective multicenter study.
Anticancer Res
; 32(8): 3119-24, 2012 Aug.
Article
em En
| MEDLINE
| ID: mdl-22843882
ABSTRACT
BACKGROUND/AIM:
Cell-free DNA may serve as a biomarker for patients with cancer; we designed our study to determine its potential in patients with bladder cancer (BCA). MATERIALS ANDMETHODS:
Short ß-actin (ACTB)-106 and large ACTB-384 fragments were quantified using real time PCR (RT-PCR); the ratio of ACTB-384/ACTB-106 was defined as DNA integrity. We analyzed the serum from 95 patients with and from 132 without BCA.RESULTS:
Patients with BCA had increased ACTB-106 levels and lower DNA integrity compared to patients without cancer. However, patients undergoing transurethral bladder resection (TURB) with histological exclusion of BCA had a similar ACTB-106 level and DNA integrity, as patients with BCA. Cell-free DNA was not correlated with smoker status, pT stage, grade or lymph node metastasis, or DNA integrity. There was a weak inverse correlation of age with DNA integrity in patients with BCA.CONCLUSION:
Analysis of serum cell-free DNA levels and fragmentation patterns are of limited value regarding the identification of patients with BCA.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Bexiga Urinária
/
DNA
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article