Iridium oxide pH sensor for biomedical applications. Case urea-urease in real urine samples.
Biosens Bioelectron
; 39(1): 163-9, 2013 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-22857994
This work demonstrates the implementation of iridium oxide films (IROF) grown on silicon-based thin-film platinum microelectrodes, their utilization as a pH sensor, and their successful formatting into a urea pH sensor. In this context, Pt electrodes were fabricated on Silicon by using standard photolithography and lift-off procedures and IROF thin films were growth by a dynamic oxidation electrodeposition method (AEIROF). The AEIROF pH sensor reported showed a super-Nerstian (72.9±0.9mV/pH) response between pH 3 and 11, with residual standard deviation of both repeatability and reproducibility below 5%, and resolution of 0.03 pH units. For their application as urea pH sensors, AEIROF electrodes were reversibly modified with urease-coated magnetic microparticles (MP) using a magnet. The urea pH sensor provided fast detection of urea between 78µM and 20mM in saline solution, in sample volumes of just 50µL. The applicability to urea determination in real urine samples is discussed.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ureia
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Urease
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Técnicas Biossensoriais
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Canavalia
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Enzimas Imobilizadas
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Irídio
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article