Quantification of endostar in rat plasma by LC-MS/MS and its application in a pharmacokinetic study.
J Pharm Biomed Anal
; 70: 505-11, 2012 Nov.
Article
em En
| MEDLINE
| ID: mdl-22858310
ABSTRACT
LC-MS/MS is a promising analytical platform for the quantification of recombinant therapeutic proteins in biological fluids for pharmacokinetic (PK) studies. Herein, an absolute quantification method based on LC-MS/MS technique was developed to quantify endostar, which is modified from the recombinant human endostatin by adding a nine-amino acid sequence (MGGSHHHHH) at the N-terminal. A reproducible three-step analytical procedure was adopted (1) Ni(2+) Sepharose was used to selectively extract endostar; (2) the signature peptide "TEAPSATGQASSLLGGR" (m/z 802.3(2+)-651.8(2+)) of endostar and a synthetic peptide "TEAPSATGQVSSLLGGR" (m/z 816.9(2+)-666.4(2+)) as internal standard (IS) were selected and analyzed in the multiple reaction monitoring (MRM) mode; (3) the proposed method was validated and applied to the pharmacokinetic study of endostar. The lower limit of quantification (LLOQ) for quantifying endostar was 50 ng/ml and this method is linear over 50-10,000 ng/ml. The accuracy was between 85% and 115%, and the intra-batch and inter-batch analytic precision and accuracy were below 15%. This LC-MS/MS approach was validated for the application to the pharmacokinetic study of endostar in rats.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida
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Inibidores da Angiogênese
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Endostatinas
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Espectrometria de Massas em Tandem
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article