ELISA with recombinant rKRP42 antigen using urine samples: a tool for predicting clinical visceral leishmaniasis cases and its outbreak.
Am J Trop Med Hyg
; 87(4): 658-62, 2012 Oct.
Article
em En
| MEDLINE
| ID: mdl-22869633
ABSTRACT
We reported a highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) that detects immunoglobulin G (IgG) in urine using rKRP42 antigen for the diagnosis of visceral leishmaniasis (VL). The ELISA was applied to study chronological change in antibody titers in five study areas in Rajshahi district, Bangladesh. A total of 585 subjects without a past VL history were examined at least three times in the 30-month follow-up period; of these subjects, 137 (23.4%) subjects became ELISA-positive at least one time during the study. Among the positive cases, 40 (29.2%) subjects developed clinical VL, and 31 (77.5%) of these subjects showed IgG titers of ≥ 1,000 U more than one time in the study period. Considering only the first ELISA results, 22 subjects with IgG titers of ≥ 1,000 U could be found, and 21 (95.5%) of these subjects turned out to be clinical cases. The high urinary IgG titers (≥ 1,000 U) will help predict possible clinical VL cases and thus, identify an outbreak in its earlier stage.
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Base de dados:
MEDLINE
Assunto principal:
Leishmania donovani
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Proteínas Recombinantes
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Imunoglobulina G
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Ensaio de Imunoadsorção Enzimática
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Surtos de Doenças
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Leishmaniose Visceral
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Antígenos de Protozoários
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article