Development of mRNA-specific RT-PCR for the detection of koi herpesvirus (KHV) replication stage.
Dis Aquat Organ
; 100(1): 11-8, 2012 Aug 13.
Article
em En
| MEDLINE
| ID: mdl-22885509
ABSTRACT
An mRNA-specific reverse transcription (RT)-PCR primer set spanning the exon junction of a spliced putative terminase gene in the koi herpesvirus (KHV) was developed to detect the replicating stage of the virus. The proposed RT-PCR amplified a target gene from the RNA template, but not from a DNA template extracted from common carp brain (CCB) cells infected with KHV. In addition, the RT-PCR did not amplify the target gene of templates extracted from specific cell lines infected with either CyHV-1 or CyHV-2. RT-PCR detected mRNA from the scales of koi experimentally infected with KHV at 24 h post exposure (hpe). However, unlike conventional PCR, RT-PCR could not detect KHV DNA in fish at 0 hpe. The results indicate that the RT-PCR developed in this study is mRNA-specific and that the assay can detect the replicating stage of KHV from both fish and cultured cells infected with the virus.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Replicação Viral
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RNA Mensageiro
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RNA Viral
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Carpas
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Herpesviridae
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article