Live imaging of primitive endoderm precursors in the mouse blastocyst.
Methods Mol Biol
; 916: 275-85, 2012.
Article
em En
| MEDLINE
| ID: mdl-22914948
ABSTRACT
The separation of two populations of cells-primitive endoderm and epiblast-within the inner cell mass (ICM) of the mammalian blastocyst is a crucial event during preimplantation development. However, many aspects of this process are still not very well understood. Recently, the identification of platelet derived growth factor receptor alpha (Pdgfrα) as an early-expressed protein that is also a marker of the later primitive endoderm lineage, together with the availability of the Pdgfra(H2B-GFP) mouse strain (Hamilton et al. Mol Cell Biol 234013-4025, 2003), has made in vivo imaging of primitive endoderm formation possible. In this chapter we present two different approaches that can be used to follow the behavior of primitive endoderm cells within the mouse blastocyst in real time.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sobrevivência de Tecidos
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Blastocisto
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Endoderma
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Imagem Molecular
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article