Sequencing and characterization of the FVB/NJ mouse genome.
Genome Biol
; 13(8): R72, 2012 Aug 23.
Article
em En
| MEDLINE
| ID: mdl-22916792
ABSTRACT
BACKGROUND:
The FVB/NJ mouse strain has its origins in a colony of outbred Swiss mice established in 1935 at the National Institutes of Health. Mice derived from this source were selectively bred for sensitivity to histamine diphosphate and the B strain of Friend leukemia virus. This led to the establishment of the FVB/N inbred strain, which was subsequently imported to the Jackson Laboratory and designated FVB/NJ. The FVB/NJ mouse has several distinct characteristics, such as large pronuclear morphology, vigorous reproductive performance, and consistently large litters that make it highly desirable for transgenic strain production and general purpose use.RESULTS:
Using next-generation sequencing technology, we have sequenced the genome of FVB/NJ to approximately 50-fold coverage, and have generated a comprehensive catalog of single nucleotide polymorphisms, small insertion/deletion polymorphisms, and structural variants, relative to the reference C57BL/6J genome. We have examined a previously identified quantitative trait locus for atherosclerosis susceptibility on chromosome 10 and identify several previously unknown candidate causal variants.CONCLUSION:
The sequencing of the FVB/NJ genome and generation of this catalog has increased the number of known variant sites in FVB/NJ by a factor of four, and will help accelerate the identification of the precise molecular variants that are responsible for phenotypes observed in this widely used strain.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Genoma
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Análise de Sequência de DNA
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Camundongos Endogâmicos C57BL
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Camundongos Endogâmicos
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article