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Characterization of vitellogenin and its derived yolk proteins in cloudy catshark (Scyliorhinus torazame).
Yamane, Kodai; Yagai, Tomoki; Nishimiya, Osamu; Sugawara, Rieko; Amano, Haruna; Fujita, Toshiaki; Hiramatsu, Naoshi; Todo, Takashi; Matsubara, Takahiro; Hara, Akihiko.
Afiliação
  • Yamane K; Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan.
Fish Physiol Biochem ; 39(2): 373-90, 2013 Apr.
Article em En | MEDLINE | ID: mdl-22918791
ABSTRACT
Elasmobranchs (sharks and rays) exhibit unique reproductive characteristics and, in contrast to the situation in teleosts, very little is known about the identity, structure and physical characteristics of their egg yolk proteins. The aims of this study were to (1) detect and purify the vitellogenin (Vtg; egg yolk precursor) and yolk proteins (YPs) of the cloudy catshark (Scyliorhinus torazame), (2) examine the relationships between Vtg and YPs and (3) characterize and classify the deduced primary structure of the Vtg transcript (vtg). The apparent molecular weights of purified Vtg and putative Vtg-related YPs (lipovitellin Lv, phosvitin Pv) were determined by gel filtration and were ~560, >669 and ~58 kDa, respectively. Following SDS-PAGE, these purified products (i.e., Vtg, Lv and Pv) appeared as bands of ~210, ~110 and ~22 kDa, respectively. On Western blots, antisera against purified Vtg, Lv and Pv recognized the ~210 kDa Vtg band. Catshark Pv, in contrast to teleost Pvs, had a very low serine content. The catshark Vtg cDNA sequence (vtg) appeared to contain an open-reading frame consisting of domains encoding Lv, Pv and ß'-component (ß'-c). A phylogenetic analysis, with a consideration of genome duplication events, placed catshark vtg into the 'vtgAB type.' It is concluded that at least a single major type of Vtg protein, which is transcribed and translated from catshark vtgAB gene, is the precursor of three egg yolk proteins (Lv, Pv and ß'-c) in catshark.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Tubarões / Proteínas do Ovo / Fosvitina / Vitelogeninas Idioma: En Ano de publicação: 2013 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Tubarões / Proteínas do Ovo / Fosvitina / Vitelogeninas Idioma: En Ano de publicação: 2013 Tipo de documento: Article