Glycolaldehyde impairs neutrophil biochemical parameters by an oxidative and calcium-dependent mechanism--protective role of antioxidants astaxanthin and vitamin C.
Diabetes Res Clin Pract
; 98(1): 108-18, 2012 Oct.
Article
em En
| MEDLINE
| ID: mdl-22921203
ABSTRACT
AIM:
The present study examined the effects of glycolaldehyde (GC) on biochemical parameters of human neutrophils and whether the antioxidant astaxanthin associated with vitamin C can modulate these parameters.METHODS:
Neutrophils from healthy subjects were treated with GC (1mM) followed or not by the antioxidants astaxanthin (2 µM) and vitamin C (100 µM). We examined the phagocytic capacity, hypochlorous acid, myeloperoxidase (MPO) and glucose-6-phosphate dehydrogenase (G6PDH) activities, cytokines and [Ca(2+)](i). Also, superoxide anion, hydrogen peroxide, nitric oxide production, antioxidant enzyme activities and glutathione-recycling system were evaluated.RESULTS:
GC promoted a marked reduction on the phagocytic capacity, maximal G6PDH and MPO activities, hypochlorous acid production and release of IL-1ß, IL-6 and TNF-α cytokines. Some impairment in the neutrophils biochemical parameters appears to be mediated by oxidative stress through ROS/RNS production and calcium reduction. Oxidative stress was evidenced by reduction in the activities of the main antioxidant enzymes, GSH/GSSG ratio and in the increment of O(2)(-) and H(2)O(2) and NO.CONCLUSIONS:
Treatment of cells with the combination of the antioxidants astaxanthin and vitamin C was able to restore some neutrophils function mainly by decreasing ROS/RNS production and improving the redox state. Overall, our findings demonstrate that GC modulates several neutrophils biochemical parameters in vitro.
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Base de dados:
MEDLINE
Assunto principal:
Ácido Ascórbico
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Membrana Celular
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Acetaldeído
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Neutrófilos
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Antioxidantes
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article