Schizandrin prevents dexamethasone-induced cognitive deficits.
Neurosci Bull
; 28(5): 532-40, 2012 Oct.
Article
em En
| MEDLINE
| ID: mdl-22961473
ABSTRACT
OBJECTIVE:
To model glucocorticoid-induced cognitive impairment and evaluate the neuroprotection by schizandrin (Sch) against dexamethasone (Dex)-induced neurotoxicity in vivo and in vitro.METHODS:
Cerebral cortical cells from neonatal Sprague-Dawley rats (within 24 hours after birth) were cultured for 9 days, and then treated with Dex (10(-4), 10(-5), 10(-6) or 10(-7) mol/L) for 24 h or pretreated with 10(-4) mol/L Dex for 24 h followed by 10, 20, 40, or 80 µmol/L Sch for 48 h. Cell viability was assessed using the MTT assay. Immunofluorescence and real-time PCR for MAP2 were performed to confirm the effects of Dex on neurite outgrowth. In vivo, kunming mice were randomly divided into six groups control [(intragastric (i.g.) vehicle for 42 days]; Dex group I (5 mg/kg · d(-1) Dex i.g. treatment for 28 days followed by i.g. vehicle for 14 days); Dex group II (Dex i.g. for 42 days); Dex + Sch (Dex i.g. for 28 days followed by 5, 15, or 45 mg/kg · d(-1) Sch i.g. for 14 days). Learning and memory were assessed by Morris water maze test. Histological examination was used to assess pathology and apoptosis in neurons.RESULTS:
Compared to the Dex groups, Sch increased cell viability in a dose-dependent manner, improved performance in the Morris water maze and ameliorated the morphological changes.CONCLUSION:
Sch has neuroprotective effects against insults induced by glucocorticoid.
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Base de dados:
MEDLINE
Assunto principal:
Compostos Policíclicos
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Dexametasona
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Transtornos Cognitivos
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Lignanas
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Fármacos Neuroprotetores
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Schisandra
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Ciclo-Octanos
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article