A simple technique to enhance the humoral immune response to intracellular protein antigens in genetic immunizations.
J Immunol Methods
; 387(1-2): 308-11, 2013 Jan 31.
Article
em En
| MEDLINE
| ID: mdl-22964556
ABSTRACT
A simple technique to enhance the humoral immune response to intracellular protein antigens in genetic immunizations is demonstrated in mice. In this approach, the intracellular protein is intentionally secreted from expressing cells as a chimeric protein, comprising an N-terminal secreted protein fused to the intracellular protein antigen. Using the Leishmania chagasi Ldccys1 cysteine protease (411CP) as an example of an intracellular protein antigen and both human and murine granulocyte colony stimulating factor (GMCSF) as examples of N-terminal secretion competent fusion partners in chimeric proteins, a humoral response in plasmid DNA immunized mice could only be detected by Western blotting when the expressed 411CP was secreted.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes de Fusão
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Proteínas
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Imunidade Humoral
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Imunogenética
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Antígenos
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article