Reversible labeling of native and fusion-protein motifs.
Nat Methods
; 9(10): 981-4, 2012 Oct.
Article
em En
| MEDLINE
| ID: mdl-22983458
ABSTRACT
The reversible covalent attachment of chemical probes to proteins has long been sought as a means to visualize and manipulate proteins. Here we demonstrate the full reversibility of post-translational custom pantetheine modification of Escherichia coli acyl carrier protein for visualization and functional studies. We use this iterative enzymatic methodology in vitro to reversibly label acyl carrier protein variants and apply these tools to NMR structural studies of protein-substrate interactions.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes de Fusão
/
Proteína de Transporte de Acila
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article