Dynamic localization of Tat protein transport machinery components in Streptomyces coelicolor.
J Bacteriol
; 194(22): 6272-81, 2012 Nov.
Article
em En
| MEDLINE
| ID: mdl-23002216
ABSTRACT
The Tat pathway transports folded proteins across the bacterial cytoplasmic membrane and is a major route of protein export in the Streptomyces genus of bacteria. In this study, we have examined the localization of Tat components in the model organism Streptomyces coelicolor by constructing enhanced green fluorescent protein (eGFP) and mCherry fusions with the TatA, TatB, and TatC proteins. All three components colocalized dynamically in the vegetative hyphae, with foci of each tagged protein being prominent at the tips of emerging germ tubes and of the vegetative hyphae, suggesting that this may be a primary site of Tat secretion. Time-lapse imaging revealed that localization of the Tat components was highly dynamic during tip growth and again demonstrated a strong preference for apical sites in growing hyphae. During aerial hypha formation, TatA-eGFP and TatB-eGFP fusions relocalized to prespore compartments, indicating repositioning of Tat components during the Streptomyces life cycle.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Membrana Transportadoras
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Proteínas de Bactérias
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Regulação Bacteriana da Expressão Gênica
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Transporte Proteico
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Streptomyces coelicolor
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article