Designing synthetic regulatory networks capable of self-organizing cell polarization.
Cell
; 151(2): 320-32, 2012 Oct 12.
Article
em En
| MEDLINE
| ID: mdl-23039994
ABSTRACT
How cells form global, self-organized structures using genetically encoded molecular rules remains elusive. Here, we take a synthetic biology approach to investigate the design principles governing cell polarization. First, using a coarse-grained computational model, we searched for all possible simple networks that can achieve polarization. All solutions contained one of three minimal motifs positive feedback, mutual inhibition, or inhibitor with positive feedback. These minimal motifs alone could achieve polarization under limited conditions; circuits that combined two or more of these motifs were significantly more robust. With these design principles as a blueprint, we experimentally constructed artificial polarization networks in yeast, using a toolkit of chimeric signaling proteins that spatially direct the synthesis and degradation of phosphatidylinositol (3,4,5)-trisphosphate (PIP(3)). Circuits with combinatorial motifs yielded clear foci of synthetic PIP(3) that can persist for nearly an hour. Thus, by harnessing localization-regulated signaling molecules, we can engineer simple molecular circuits that reliably execute spatial self-organized programs.
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Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Fosfatos de Fosfatidilinositol
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Modelos Biológicos
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article