Localisation of a family of complex-forming ß-barrels in the T. vaginalis hydrogenosomal membrane.
FEBS Lett
; 586(22): 4038-45, 2012 Nov 16.
Article
em En
| MEDLINE
| ID: mdl-23068613
ABSTRACT
Crucial to organellogenesis was the development of membrane translocases responsible for delivering proteins to new cellular compartments. This investigation examines the Trichomonas vaginalis hydrogenosome, a mitochondrially derived organelle. We identify an expanded family of putative ß-barrel proteins (THOM A-I) comprising nine related sequences. Sub-cellular localisation by immunofluorescence and biochemical fractionation is consistent with THOMs being localised to the hydrogenosomal membrane. Native gel electrophoresis and chemical cross-linking support the ability of THOM proteins to be components of membrane-bound oligomeric protein complexes, consistent with a role in protein translocation.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Membrana Transportadoras
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Trichomonas vaginalis
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Organelas
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Proteínas de Protozoários
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Membranas Intracelulares
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article