Functional annotation and ENU.
BMC Res Notes
; 5: 580, 2012 Oct 24.
Article
em En
| MEDLINE
| ID: mdl-23095518
ABSTRACT
Functional annotation of every gene in the mouse genome is a herculean task that requires a multifaceted approach. Many large-scale initiatives are contributing to this undertaking. The International Knockout Mouse Consortium (IKMC) plans to mutate every protein-coding gene, using a combination of gene trapping and gene targeting in embryonic stem cells. Many other groups are performing using the chemical mutagen ethylnitrosourea (ENU) or transpon-based systems to induce mutations, screening offspring for phenovariants and identifying the causative mutations. A recent paper in BMC Research Notes by Arnold et al. presents data from an ENU-based mutagenesis project that provides not only some of the first phenotype-genotype information for a large number of genes, but also a trove of information, all publicly available, that demonstrates the specificity and efficiency of ENU mutagenesis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Etilnitrosoureia
/
Mutagênicos
/
Mutação
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article