Association of TLR5 sequence variants and mRNA level with cytokine transcription in pigs.
Immunogenetics
; 65(2): 125-32, 2013 Feb.
Article
em En
| MEDLINE
| ID: mdl-23132291
ABSTRACT
The Toll-like receptor 5 (TLR5) plays a crucial role in host defense against flagellated bacteria by recognizing flagellin. Accumulating evidence suggests that single nucleotide polymorphisms (SNPs) in TLR5 have an effect on flagellin recognition and are associated with susceptibility/resistance to disease. In this study, we analyzed association of SNPs, including c.834T>G, c.1065T>C, c.1205C>T, c.1246A>T, c.1269G>A, and c.1398C>T, as well as mRNA level of TLR5 with the abundance of transcripts of cytokines in pigs. SNPs c.1246A>T and c.1269G>A were significantly associated with the transcript abundance of interleukin (IL)-2, and SNPs c.834T>G and c.1398C>T with IL-10 (P < 0.05); the haplotypes showed a tendency to affect the transcript abundance of IL-10 (P = 0.0660) and significantly associated with the transcription of TLR5 (P < 0.01); the abundance of transcripts of TLR5 and IL-10 were strongly correlated (P < 0.01). The results indicated that the SNPs, associated with the transcript abundance of cytokines, were related to immune responsiveness mediated by cytokine, which, in turn, would have a role in pig breeding for disease resistance. Furthermore, the positive correlation between the abundance of TLR5 and IL10 suggest a link between TLR5 activation and IL-10 expression in porcine.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Transcrição Gênica
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RNA Mensageiro
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Citocinas
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Polimorfismo de Nucleotídeo Único
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Receptor 5 Toll-Like
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article