[Alisol B inhibited complement 3a-induced human renal tubular epithelial to mesenchymal transition].
Zhongguo Zhong Xi Yi Jie He Za Zhi
; 32(10): 1407-12, 2012 Oct.
Article
em Zh
| MEDLINE
| ID: mdl-23163157
ABSTRACT
OBJECTIVE:
To study whether alisol B could inhibit complement 3a (C3a) induced renal tubular epithelial-mesenchymal transition (EMT).METHODS:
The in vitro cultured human renal tubular epithelial HK-2 cells were intervened with 5 ng/mL transforming growth factor-beta (TGF-beta), 0.1 micromol C3a, and 0.1 micromol C3a + 10 micromol alisol B, respectively. The mRNA and protein expressions of alpha-SMA, E-cadherin, and C3 were detected using RT-PCR, Western blot, and immunofluorescence, respectively.RESULTS:
The mRNA and protein expressions of C3 in HK-2 cells were up-regulated after intervention of C3a (P < 0.01), the mRNA and protein expressions of alpha-SMA in HK-2 cells were obviously enhanced (P < 0.01), the mRNA and protein expressions of E-cadherin obviously decreased (P < 0.01). When compared with the group intervened by exogenous C3a, after intervention of alisol B, the mRNA and protein expressions of alpha-SMA in HK-2 cells were obviously reduced (P < 0.01), the mRNA and protein expressions of E-cadherin obviously increased (P < 0.05).CONCLUSIONS:
Exogenous C3a could induce renal tubular EMT. Alisol B was capable of suppressing C3a induced EMT.
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Base de dados:
MEDLINE
Assunto principal:
Colestenonas
/
Células Epiteliais
/
Transição Epitelial-Mesenquimal
/
Túbulos Renais
Idioma:
Zh
Ano de publicação:
2012
Tipo de documento:
Article