Development of new polymorphic microsatellite loci for the barley stem gall midge, Mayetiola hordei (Diptera: Cecidomyiidae) from an enriched library.
Int J Mol Sci
; 13(11): 14446-50, 2012 Nov 08.
Article
em En
| MEDLINE
| ID: mdl-23203074
ABSTRACT
Using an enriched library method, seven polymorphic microsatellite loci were isolated from the barley stem gall midge, Mayetiola hordei. Polymorphism at loci was surveyed on 57 individual midges collected on barley in Tunisia. Across loci, polymorphism ranged from two to six alleles per locus. The observed heterozygosity varied between 0.070 and 0.877. Based on the number of alleles detected and the associated levels of heterozygosity, we believe that these loci will prove useful for population genetic studies on M. hordei.
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Base de dados:
MEDLINE
Assunto principal:
Polimorfismo Genético
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Hordeum
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Caules de Planta
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Repetições de Microssatélites
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Loci Gênicos
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article