Role of TLR-2 and fungal surface antigens on innate immune response against Sporothrix schenckii.
Immunol Invest
; 42(1): 36-48, 2013.
Article
em En
| MEDLINE
| ID: mdl-23231043
ABSTRACT
Sporotrichosis is an infection caused by the dimorphic fungus Sporothrix schenckii. Toll-like receptors (TLRs) play an important role in immunity, since they bind to pathogen surface antigens and initiate the immune response. However, little is known about the role of TLR-2 and fungal surface antigens in the recognition of S. schenckii and in the subsequent immune response. This study aimed to evaluate the involvement of TLR-2 and fungal surface soluble (SolAg) and lipidic (LipAg) antigens in phagocytosis of S. schenckii and production of immune mediators by macrophages obtained from WT and TLR-2(-/-) animals. The results showed that TLR-2(-/-) animals had had statistical lower percentage of macrophages with internalized yeasts compared to WT. SolAg and LipAg impaired phagocytosis and immunological mediator production for both WT and TLR-2(-/-). The absence of TLR-2 led to lower production of the cytokines TNF-α, IL-1ß, IL-12 and IL-10 compared to WT animals. These results suggest a new insight in relation to how the immune system, through TLR-2, recognizes and induces the production of mediators in response to the fungus S. schenckii.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Esporotricose
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Sporothrix
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Receptor 2 Toll-Like
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Macrófagos
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Antígenos de Fungos
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article