[Effect of Fasudil on miroglia and astrocytes in experimental autoimmune encephalomyelitis mice].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
; 28(12): 1242-5, 2012 Dec.
Article
em Zh
| MEDLINE
| ID: mdl-23232512
ABSTRACT
AIM:
To explore the therapeutic effect of Fasudil and its possible mechanisms in experimental autoimmune encephalomyelitis (EAE) mice, mainly focusing on the roles of microglia and astrocytes in the treatment.METHODS:
Female adult C57BL/6 mice were immunized with MOG35-55 to induce chronic EAE. Fasudil was injected on day 3 p.i. (early Fasudil treatment), or at the onset of EAE (late Fasudil treatment). Normal saline was injected in other mice as EAE controls in a similar manner. Clinical score and body mass were recorded every other day. The expressions of iNOS on microglia and p-NF-κB/p65 on astrocytes were measured by immunohistochemistry and Western blotting. The levels of IL-1ß and TNF-α in spinal cord homogenate were determined by ELISA.RESULTS:
Fasudil delayed onset and ameliorated the severity of EAE. Fasudil inhibited the expression of iNOS on microglia and p-NF-κB/p65 on astrocytes in spinal cords, accompanied by the inhibition of inflammatory factors IL-1ß and TNF-α.CONCLUSION:
Fasudil exhibits therapeutic effect on EAE, possibly through inhibiting inflammatory molecules on microglia and astrocyte.
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Base de dados:
MEDLINE
Assunto principal:
Neuroglia
/
Astrócitos
/
1-(5-Isoquinolinasulfonil)-2-Metilpiperazina
/
Encefalomielite Autoimune Experimental
Idioma:
Zh
Ano de publicação:
2012
Tipo de documento:
Article