Studying the activation of epithelial ion channels using global whole-field photolysis.
Cold Spring Harb Protoc
; 2013(1)2013 Jan 01.
Article
em En
| MEDLINE
| ID: mdl-23282643
ABSTRACT
The production of saliva by parotid acinar cells is stimulated by Ca(2+) activation of Cl(-) and K(+) channels located in the apical plasma membrane of these polarized cells. Here we provide a detailed description of a flash photolysis experiment designed to give a global and relatively uniform photorelease of inositol 1,4,5-trisphosphate (InsP(3)) or Ca(2+) from caged precursors (NPE-InsP(3) or NP-EGTA) combined with the simultaneous measurement of whole-cell Ca(2+)-activated currents. The photolysis light source can be either an ultraviolet (UV) flash lamp or alternatively the output from a 375-nm diode laser, which is defocused to illuminate the entire field.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fotólise
/
Técnicas Citológicas
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Células Acinares
/
Canais Iônicos
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article