PARP-2 regulates cell cycle-related genes through histone deacetylation and methylation independently of poly(ADP-ribosyl)ation.
Biochem Biophys Res Commun
; 431(1): 58-64, 2013 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-23291187
ABSTRACT
Poly(ADP-ribose) polymerase-2 (PARP-2) catalyzes poly(ADP-ribosyl)ation (PARylation) and regulates numerous nuclear processes, including transcription. Depletion of PARP-2 alters the activity of transcription factors and global gene expression. However, the molecular action of how PARP-2 controls the transcription of target promoters remains unclear. Here we report that PARP-2 possesses transcriptional repression activity independently of its enzymatic activity. PARP-2 interacts and recruits histone deacetylases HDAC5 and HDAC7, and histone methyltransferase G9a to the promoters of cell cycle-related genes, generating repressive chromatin signatures. Our findings propose a novel mechanism of PARP-2 in transcriptional regulation involving specific protein-protein interactions and highlight the importance of PARP-2 in the regulation of cell cycle progression.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Poli Adenosina Difosfato Ribose
/
Histonas
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Ciclo Celular
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Regulação da Expressão Gênica
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Poli(ADP-Ribose) Polimerases
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article