Identification of DNA-dependent protein kinase catalytic subunit as a novel interaction partner of lymphocyte enhancer factor 1.
Med Mol Morphol
; 46(1): 14-9, 2013 Mar.
Article
em En
| MEDLINE
| ID: mdl-23325550
ABSTRACT
Lymphocyte enhancer factor 1 (LEF1), a member of the LEF/T-cell-specific factor (TCF) family of the high mobility group domain transcription factors, acts downstream in canonical Wnt signaling. Aberrant transactivation of LEF1 contributes to the tumorigenesis of colonic neoplasms, sebaceous skin tumors, and lymphoblastic leukemia. LEF1-associated proteins are crucial for regulating its transcriptional activity. In this study, glutathione-S-transferase pull-down assay and mass spectrometry enabled identification of the DNA-dependent protein kinase catalytic subunit (DNA-PKcs) as a novel interaction partner for LEF1. The interaction between LEF1 and DNA-PKcs was confirmed using in vivo co-immunoprecipitation. Furthermore, double immunofluorescence observations showed that LEF1 and DNA-PKcs colocalized in the nuclei of colon adenocarcinoma cell lines. Identification of the interaction between LEF1 and DNA-PKcs may provide clues for a novel therapy for cancer treatment as well as for understanding LEF1-mediated transcriptional regulation.
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Base de dados:
MEDLINE
Assunto principal:
Adenocarcinoma
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Regulação Neoplásica da Expressão Gênica
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Neoplasias do Colo
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Proteína Quinase Ativada por DNA
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Fator 1 de Ligação ao Facilitador Linfoide
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Via de Sinalização Wnt
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article