Surface-enhanced Raman scattering-based sensing in vitro: facile and label-free detection of apoptotic cells at the single-cell level.
Anal Chem
; 85(5): 2809-16, 2013 Mar 05.
Article
em En
| MEDLINE
| ID: mdl-23373817
ABSTRACT
Surface-enhanced Raman scattering (SERS) is well recognized as a powerful analytical tool, enabling ultrahigh sensitive detection of analytes at low concentrations, even down to single-molecule level. Of particular note, in comparison to sufficient investigations on SERS-based detection of biomolecules (e.g., DNA and protein), there has been relatively scanty information regarding in vitro and in vivo detection. In this Article, we demonstrate a kind of SERS-active substrate, i.e., AgNPs-decorated silicon wafer (AgNPs@Si), as a high-performance in vitro sensing platform for single-cell detection of apoptotic cells. The AgNPs@Si yields highly reproducible SERS signals with an enhancement factor of â¼10(7). Remarkably, cellular experiments show that facile, noninvasive, label-free, and sensitive detection of apoptotic cells is readily realized using the high-performance SERS-active platform. Three kinds of apoptotic cells treated with apoptosis inducer are facilely and sensitively detected at the single-cell level, suggesting the exciting potential of AgNPs@Si for SERS-based in vitro analysis and detection.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Análise Espectral Raman
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Separação Celular
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Apoptose
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Imagem Molecular
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Análise de Célula Única
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article