Endothelial cells promote the colorectal cancer stem cell phenotype through a soluble form of Jagged-1.
Cancer Cell
; 23(2): 171-85, 2013 Feb 11.
Article
em En
| MEDLINE
| ID: mdl-23375636
ABSTRACT
We report a paracrine effect whereby endothelial cells (ECs) promote the cancer stem cell (CSC) phenotype of human colorectal cancer (CRC) cells. We showed that, without direct cell-cell contact, ECs secrete factors that promoted the CSC phenotype in CRC cells via Notch activation. In human CRC specimens, CD133 and Notch intracellular domain-positive CRC cells colocalized in perivascular regions. An EC-derived, soluble form of Jagged-1, via ADAM17 proteolytic activity, led to Notch activation in CRC cells in a paracrine manner; these effects were blocked by immunodepletion of Jagged-1 in EC-conditioned medium or blockade of ADAM17 activity. Collectively, ECs play an active role in promoting Notch signaling and the CSC phenotype by secreting soluble Jagged-1.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco Neoplásicas
/
Proteínas de Ligação ao Cálcio
/
Neoplasias Colorretais
/
Peptídeos e Proteínas de Sinalização Intercelular
/
Células Endoteliais
/
Receptores Notch
/
Neoplasias Hepáticas
/
Proteínas de Membrana
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article