Volatile fingerprints of cancer specific genetic mutations.
Nanomedicine
; 9(6): 758-66, 2013 Aug.
Article
em En
| MEDLINE
| ID: mdl-23428987
ABSTRACT
We report on a new concept for profiling genetic mutations of (lung) cancer cells, based on the detection of patterns of volatile organic compounds (VOCs) emitted from cell membranes, using an array of nanomaterial-based sensors. In this in-vitro pilot study we have derived a volatile fingerprint assay for representative genetic mutations in cancer cells that are known to be associated with targeted cancer therapy. Five VOCs were associated with the studied oncogenes, using complementary chemical analysis, and were discussed in terms of possible metabolic pathways. The reported approach could lead to the development of novel methods for guiding treatments, so that patients could benefit from safer, more timely and effective interventions that improve survival and quality of life while avoiding unnecessary invasive procedures. Studying clinical samples (tissue/blood/breath) will be required as next step in order to determine whether this cell-line study can be translated into a clinically useful tool. FROM THE CLINICAL EDITOR In this novel study, a new concept for profiling genetic mutations of (lung) cancer cells is described, based on the detection of patterns of volatile organic compounds emitted from cell membranes, using an array of nano-gold based sensors.
Palavras-chave
ALK; Anaplastic lmphoma kinase; CV1; DFA; Discriminant factor analysis; EGFR; EGFRmut; EML4; EML4-ALK; Echinoderm microtubule-associated protein-like 4; Epidermal growth factor receptor; First canonical variable; GC-MS; GNP; Gas-chromatography/mass-spectrometry; Genetic; Gold nanoparticle; KRAS; KRASmut; LC; Lung cancer; Mutation; NSCLC; Non-small cell lung carcinoma; Sensor; TKI; Tyrosine kinase inhibitor; V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog; VOC; Volatile organic compound; Wild type; fusion of the EML4 gene to the ALK gene; mutated EGFR gene; mutated KRAS gene; wt
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Compostos Orgânicos Voláteis
/
Neoplasias Pulmonares
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article