A mini-intronic plasmid (MIP): a novel robust transgene expression vector in vivo and in vitro.
Mol Ther
; 21(5): 954-63, 2013 May.
Article
em En
| MEDLINE
| ID: mdl-23459514
ABSTRACT
The bacterial backbone (BB) sequences contained within a canonical plasmid DNA dampen exogenous transgene expression by tenfold to 1,000-fold over a period of a few weeks following transfection into quiescent tissues such as the liver. Minicircle DNA vectors devoid of bacterial plasmid backbone sequences overcome transgene silencing providing persistent transgene expression. Because, we recently established that the length rather than sequence of the DNA flanking the transgene expression cassette is the major parameter affecting transgene silencing, we developed an alternative plasmid propagation process in which the essential bacterial elements for plasmid replication and selection are placed within an engineered intron contained within the eukaryotic expression cassette. As with the minicircle vector, the mini-intronic plasmid (MIP) vector system overcomes transgene silencing observed with plasmids but in addition provides between 2 and often 10 times or higher levels of transgene expression compared with minicircle vectors containing the same expression cassette in vivo and in vitro. These improved plasmids will benefit all studies involving gene transfer/therapy approaches.
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Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Íntrons
/
Expressão Gênica
/
Transgenes
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article