Mass spectrometry identification of granins and other proteins secreted by neuroblastoma cells.
Tumour Biol
; 34(3): 1773-81, 2013 Jun.
Article
em En
| MEDLINE
| ID: mdl-23519838
ABSTRACT
We used mass spectrometry-based protein identification to determine the presence of granins and other proteins in the mouse neuroblastoma secretome. We detected polypeptides derived from four members of the granin family chromogranin A, chromogranin B, secretogranin III, and VGF. Many of them are derived from previously described biologically active regions; however, for VGF and CgB, we detected peptides not related to known bioactivities. Along with granins, we identified 115 other proteins secreted by mouse neuroblastoma cells, belonging to different functional categories. Fifty-six out of 119 detected proteins possess the signal fragments required for translocation into endoplasmic reticulum. Sequences of remaining 63 proteins were analyzed using SecretomeP algorithm to determine probability of nonclassical secretion. Identified proteins are involved in the regulation of cell cycle, proliferation, apoptosis, angiogenesis, proteolysis, and cell adhesion.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fragmentos de Peptídeos
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Proteínas
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Biomarcadores Tumorais
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Cromograninas
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Proteoma
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Espectrometria de Massas em Tandem
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Neuroblastoma
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article