Crystal structure of SsfS6, the putative C-glycosyltransferase involved in SF2575 biosynthesis.
Proteins
; 81(7): 1277-82, 2013 Jul.
Article
em En
| MEDLINE
| ID: mdl-23526584
ABSTRACT
The molecule known as SF2575 from Streptomyces sp. is a tetracycline polyketide natural product that displays antitumor activity against murine leukemia P388 in vivo. In the SF2575 biosynthetic pathway, SsfS6 has been implicated as the crucial C-glycosyltransferase (C-GT) that forms the C-C glycosidic bond between the sugar and the SF2575 tetracycline-like scaffold. Here, we report the crystal structure of SsfS6 in the free form and in complex with TDP, both at 2.4 Å resolution. The structures reveal SsfS6 to adopt a GT-B fold wherein the TDP and docked putative aglycon are consistent with the overall C-glycosylation reaction. As one of only a few existing structures for C-glycosyltransferases, the structures described herein may serve as a guide to better understand and engineer C-glycosylation.
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Base de dados:
MEDLINE
Assunto principal:
Tetraciclinas
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Proteínas de Bactérias
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Cristalografia por Raios X
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Antibacterianos
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article