Detection of base excision repair enzyme activity using a luminescent G-quadruplex selective switch-on probe.
Chem Commun (Camb)
; 49(50): 5630-2, 2013 Jun 25.
Article
em En
| MEDLINE
| ID: mdl-23559154
ABSTRACT
We report herein a simple and convenient luminescent assay for detection of base excision repair enzyme activity using an Ir(III) complex as a G-quadruplex selective probe. Using uracil-DNA glycosylase (UDG) as a model enzyme, the assay achieved high sensitivity and selectivity for UDG over other tested enzymes. The utility of the assay for screening potential UDG inhibitors was also demonstrated.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Uracila-DNA Glicosidase
/
Quadruplex G
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article