Protein chemical synthesis by serine and threonine ligation.
Proc Natl Acad Sci U S A
; 110(17): 6657-62, 2013 Apr 23.
Article
em En
| MEDLINE
| ID: mdl-23569249
ABSTRACT
An efficient method has been developed for the salicylaldehyde ester-mediated ligation of unprotected peptides at serine (Ser) or threonine (Thr) residues. The utility of this peptide ligation approach has been demonstrated through the convergent syntheses of two therapeutic peptides--ovine-corticoliberin and Forteo--and the human erythrocyte acylphosphatase protein (â¼11 kDa). The requisite peptide salicylaldehyde ester precursor is prepared in an epimerization-free manner via Fmoc-solid-phase peptide synthesis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
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Hormônio Liberador da Corticotropina
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Engenharia de Proteínas
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Hidrolases Anidrido Ácido
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Teriparatida
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Aldeídos
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article