Polymerase chain reaction to detect hepatitis B virus DNA and RNA sequences in primary liver cancers from patients negative for hepatitis B surface antigen.
N Engl J Med
; 323(2): 80-5, 1990 Jul 12.
Article
em En
| MEDLINE
| ID: mdl-2359427
ABSTRACT
BACKGROUND AND METHODS:
The role of hepatitis B virus (HBV) in the course of patients with primary liver cancer who are negative for hepatitis B surface antigen has been debated. We used the polymerase chain reaction to evaluate 28 such patients for the presence of DNA and RNA sequences of the virus; 22 of these patients had associated cirrhosis. The patients were from areas with different prevalences of HBV infection (South Africa, Italy, France, and Japan).RESULTS:
Antibodies to the surface and core antigens of HBV were detected in 10 of the 23 patients tested. HBV DNA sequences were detected in 17 of the 28 patients, including 8 of the 10 with HBV antibodies and 6 of 13 without HBV serologic markers. HBV RNA molecules were found in four of five tumors tested.CONCLUSIONS:
Our investigation indicates that transcriptionally active HBV genomes are present in various geographic areas among patients with liver cancer who are negative for hepatitis B surface antigen. This observation is consistent with an etiologic role for the virus in the development of these tumors.
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Base de dados:
MEDLINE
Assunto principal:
DNA Viral
/
RNA Viral
/
Vírus da Hepatite B
/
Antígenos de Superfície da Hepatite B
/
Neoplasias Hepáticas
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article