A rapid DNA assembling strategy mediated by direct full-length polymerase chain reaction.
Gene
; 523(2): 122-5, 2013 Jul 10.
Article
em En
| MEDLINE
| ID: mdl-23603018
ABSTRACT
An efficient DNA assembling strategy was developed here modified from Class-IIS endonuclease mediated DNA splicing by directed ligation (SDL). Benefited from the full-length PCR directly using ligation products as template, this strategy required less effort and less time to obtain the assembled full-length DNA. The advantages of this strategy made it a rapid and easy-to-perform gene splicing and multiple site-directed mutagenesis approach especially practicable when more fragments need to be assembled at the same time.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Reação em Cadeia da Polimerase
/
Clonagem Molecular
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article