Antibodies to cannabinoid type 1 receptor co-react with stomatin-like protein 2 in mouse brain mitochondria.
Eur J Neurosci
; 38(3): 2341-8, 2013 Aug.
Article
em En
| MEDLINE
| ID: mdl-23617247
ABSTRACT
Anti-cannabinoid type 1 receptor (CB1 ) polyclonal antibodies are widely used to detect the presence of CB1 in a variety of brain cells and their organelles, including neuronal mitochondria. Surprisingly, we found that anti-CB1 sera, in parallel with CB1 , also recognize the mitochondrial protein stomatin-like protein 2. In addition, we show that the previously reported effect of synthetic cannabinoid WIN 55,212-2 on mitochondrial complex III respiration is not detectable in purified mitochondrial preparations. Thus, our study indicates that a direct relationship between endocannabinoid signaling and mitochondrial functions in the cerebral cortex seems unlikely, and that caution should be taken interpreting findings obtained using anti-CB1 antibodies.
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MEDLINE
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Proteínas Mitocondriais
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Receptor CB1 de Canabinoide
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Soros Imunes
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Proteínas de Membrana
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Proteínas do Tecido Nervoso
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article