Experimental determination of water equilibration rates in the hanging drop method of protein crystallization.
Anal Biochem
; 186(2): 332-9, 1990 May 01.
Article
em En
| MEDLINE
| ID: mdl-2363507
ABSTRACT
The hanging drop method for protein crystallization consists of equilibrating a water droplet containing the protein and a precipitant against a reservoir solution at a higher precipitant concentration. The time for water equilibration--which affects the kinetics of crystallization--to reach 90% of completion is shown to vary between about 25 h and more than 25 days, depending on experimental conditions. Experiments were performed with three of the most widely used precipitants (ammonium sulfate, polyethylene glycol, 2-methyl-2,4-pentanediol), combining various parameters expected to affect the rate of water evaporation. The most dramatic effects were obtained when varying temperature and initial drop volume. A simple empirical equation for estimating the kinetics of water equilibration under given crystallization conditions is proposed.
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Base de dados:
MEDLINE
Assunto principal:
Água
/
Proteínas
/
Cristalização
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article