Study on urinary profile of inborn errors of metabolism by 18-crown-6 modified capillary electrophoresis with laser-induced fluorescence detection.
J Chromatogr B Analyt Technol Biomed Life Sci
; 929: 102-6, 2013 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-23669610
ABSTRACT
Newborn screening in urine is important for the diagnosis of many inborn errors of metabolism (IEM). Capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) is a major technological advance in screening IEM. It has the advantage of sensitive and simultaneous multiple disease screening with minimal sample requirement. The analytes were derivatized with fluorescein isothiocyanate (FITC) prior to CE-LIF analysis. In urine samples, free amino acids (AAs) were well separated from other coexisting components, exhibiting a linear calibration over the concentration range 0.01-5.0µmol/L with the limits of detection (LODs) ranging from 0.005 to 0.010µmol/L. The relative standard deviations (RSDs) were in the range 0.1-1.0% for peak area, and 0.2-1.0% for migration time, respectively. Under optimized conditions, the method presented here has been successfully used for the simultaneous and sensitive analysis of seven AAs in urine samples of newborn babies, and evaluating the effect of therapy as well.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Fluorescência
/
Eletroforese Capilar
/
Éteres de Coroa
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Lasers
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Erros Inatos do Metabolismo
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article