Probing deep into the interaction of a fluorescent chalcone derivative and bovine serum albumin (BSA): an experimental and computational study.
Org Biomol Chem
; 11(29): 4764-77, 2013 Aug 07.
Article
em En
| MEDLINE
| ID: mdl-23680860
ABSTRACT
In the present manuscript, a novel fluorescent chalcone derivative is synthesized and its photophysical properties are fully characterized. The designed fluorophore is applied as a probe to study protein-dye interactions with bovine serum albumin. Circular dichroism gave interesting results on the thermodynamics of the interaction. NMR spectroscopy, especially relaxation measurements, revealed the atoms in the chalcone derivative that interacts with the protein upon binding. Molecular docking calculations indicate that the most favourable binding sites are near the two tryptophan residues. Furthermore, ab initio and DFT calculations offer insights into the reactivity and physicochemical properties of this novel fluorophore.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Teoria Quântica
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Soroalbumina Bovina
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Chalcona
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Corantes Fluorescentes
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article