Analyzing plant signaling phospholipids through 32Pi-labeling and TLC.
Methods Mol Biol
; 1009: 3-15, 2013.
Article
em En
| MEDLINE
| ID: mdl-23681518
ABSTRACT
Lipidomic analyses through LC-, GC-, and ESI-MS/MS can detect numerous lipid species based on headgroup and fatty acid compositions but usually miss the minor phospholipids involved in cell signaling because of their low chemical abundancy. Due to their high turnover, these signaling lipids are, however, readily picked up by labeling plant material with (32)P-orthophosphate and subsequent analysis of the lipid extracts by thin layer chromatography. Here, protocols are described for suspension-cultured tobacco BY-2 cells, young Arabidopsis seedlings, Vicia faba roots, and Arabidopsis leaf disks, which can easily be modified for other plant species and tissues.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fosfolipídeos
/
Plantas
/
Transdução de Sinais
/
Cromatografia em Camada Fina
/
Arabidopsis
/
Marcação por Isótopo
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article