Microbial tracking of multidrug-resistant Klebsiella pneumoniae isolates in a pediatric hospital setting.
Int J Immunopathol Pharmacol
; 26(2): 463-72, 2013.
Article
em En
| MEDLINE
| ID: mdl-23755761
ABSTRACT
We investigated the clonal relatedness of seven multi-drug-resistant (MDR) Klebsiella pneumoniae isolates, as well as three susceptible K. pneumoniae isolates collected during hospital outbreaks and outbreak-related microbiological surveillance, respectively. The relatedness among K. pneumoniae isolates was assessed by pulsed field gel electrophoresis (PFGE) and automated repetitive-sequence-based PCR (rep-PCR) genotyping and the results were compared to a proteomic phenotyping performed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). All typing methods agreed on the generation of three different clusters of K. pneumoniae isogenetic/related MDR strains. After strengthening hospital infection control measures, no other spreading events involving MDR-K. pneumoniae were reported until the end of the observation period. This preliminary investigation suggests that, in a hierarchical approach to bacterial typing, MALDI-TOF MS proteome profiling might offer a fast and valuable preliminary screening tool able to support microbiologists during nosocomial outbreak surveys.
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Base de dados:
MEDLINE
Assunto principal:
Infecções por Klebsiella
/
Infecção Hospitalar
/
Surtos de Doenças
/
Farmacorresistência Bacteriana Múltipla
/
Hospitais Pediátricos
/
Klebsiella pneumoniae
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article