Capturing protein-coding genes across highly divergent species.
Biotechniques
; 54(6): 321-6, 2013 Jun.
Article
em En
| MEDLINE
| ID: mdl-23758502
ABSTRACT
DNA hybridization capture combined with next generation sequencing can be used to determine the sequences of hundreds of target genes across hundreds of individuals in a single experiment. However, the approach has thus far only been successfully applied to capture targets that are highly similar in sequence to the bait molecules. Here we introduce modifications that extend the reach of the method to allow efficient capture of highly divergent homologous target sequences using a single set of baits. These modifications have important implications for comparative biology.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Genoma
/
Análise de Sequência de DNA
/
Genes
/
Hibridização de Ácido Nucleico
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article