Changes in the phospholipid fatty acid composition of the lipid droplet during the differentiation of 3T3-L1 adipocytes.
J Biochem
; 154(3): 281-9, 2013 Sep.
Article
em En
| MEDLINE
| ID: mdl-23760554
ABSTRACT
Lipid droplets (LDs) are independent organelles in adipocytes that are composed of a lipid ester core surrounded by a phospholipid monolayer. The fatty acid composition of the phospholipid monolayer should determine the metabolism and dynamics of LDs. In this study, we examined the fatty acid composition of phospholipid monolayer in LDs during the differentiation of 3T3-L1 adipocytes. The levels of saturated fatty acids (SFAs), such as 160 and 180, in phosphatidylcholine (PC) and phosphatidylethanolamine (PE) of LDs decreased during differentiation. In contrast, the levels of monounsaturated fatty acids (MUFAs) such as 161n-7 and 181n-9 in PC and PE of LDs and the level of polyunsaturated fatty acids (PUFAs) such as 203n-6, 204n-6 and 226n-3 in PE of LDs increased during differentiation. These results suggest that the phospholipid monolayer in mature LDs is more fluid than that in nascent LDs. The fatty acid compositions of the LD monolayer were different from those of the microsome bilayer in the early stage of differentiation, but similar to those of the microsome bilayer in the late stage of differentiation. These data provide evidence that biophysical properties of the phospholipid monolayer in LDs change during adipocyte differentiation.
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Base de dados:
MEDLINE
Assunto principal:
Organelas
/
Diferenciação Celular
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Adipócitos
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Metabolismo dos Lipídeos
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article