Chitosan conjugates for DNA delivery.
Phys Chem Chem Phys
; 15(28): 11893-9, 2013 Jul 28.
Article
em En
| MEDLINE
| ID: mdl-23764856
ABSTRACT
Graft copolymers of chitosan and maltodextrin were synthesized by reductive amination of a low molecular weight chitosan. The degree of substitution is 70% or above, as quantified by nuclear magnetic resonance spectroscopy, at molar ratios of chitosan glucosamine units and maltodextrin of 1 1 and 1 5. The high substitution degree generates a water-soluble compound at pH 7.4 by reducing the amino groups of chitosan. In addition, the copolymer self-assembles into spherical nanoparticles with diameters of about 300 nm. The mass polymer/DNA ratios at the isoelectric point are within the range of 3-3.5 for chitosan-maltodextrin nanoparticles as determined by electrophoretic mobility measurements. These results confirm that DNA interacts efficiently with the copolymer nanoparticles indicating a potential application of the system for DNA delivery.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Portadores de Fármacos
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Quitosana
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article