WUSCHEL-responsive At5g65480 interacts with CLAVATA components in vitro and in transient expression.
PLoS One
; 8(6): e66345, 2013.
Article
em En
| MEDLINE
| ID: mdl-23776660
ABSTRACT
The CLAVATA (CLV) signaling pathway is essential for shoot meristem homeostasis in Arabidopsis. CLV acts to limit the expression domain of the stem cell-promoting gene WUSCHEL (WUS). The closely related receptor-kinases CLV1 and BAM1 are key components in this pathway; however, the downstream factors that link the receptors to WUS regulation are poorly understood. The Arabidopsis gene At5g65480 was recently identified as a direct transcriptional target up-regulated by WUS. We have independently identified this gene which we term CCI1 as a CLV1 and BAM1 interacting protein in vitro and in transient expression. CCI1 has phosphatidylinositide-binding activity in vitro and localizes to the plasma membrane in transient expression. Furthermore, CLV signaling components and CCI1 both partition to detergent-resistant membrane microdomains characterized as lipid rafts.
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Base de dados:
MEDLINE
Assunto principal:
Arabidopsis
/
Proteínas de Homeodomínio
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Proteínas de Arabidopsis
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article